3/24/2024 0 Comments Fiji imagej mp4The reason histogram matching cannot be used for the measurement, to explain in your case, is because the algorithm assumes that the histogram shape is always constant (which also means that the average intensity is constant over time). On behalf of Diego Barcena (Mark Isalan group, CRG, Barcelona) Xavier Sanjuan (ALMU, Parc de Recerca Biomèdica de Barcelona), Other thing is at this moment it is difficult to know is wherther everything is bleached (so GFP signal kept constant reflects an increase) or wherther bleaching affects only the medium (so GFP is really constant and is not increasing, which is not what he expects…). The thing is that, as you mention in your blog's entry (, ), with this method you cannot quantify intensities.Ĭan you recommend us an alternative method to be able to quantify changes in the GFP signal over time? He obtains the best visualization of what he expects with the Histogram Matching Method. To compensate background bleaching he is using your bleach_corrector plugin in FIJI. He really expects the GFP to increase over the time, and he would like to quantify this increase in GFP signal over time. Over the time, background intensity decreases while specific signal keeps more or less the same so it becomes gradually more visible. GFP signal is very dim and background is quite strong (so SNR very poor). One of our users is making timelapse experiments to track a GFP marker in cell cultures.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |